منابع مشابه
A CRISPR / Cas 9 toolbox for
Running title: A CRISPR/Cas9 toolbox for gene editing and transcriptional regulation 1 2 3 Co-corresponding authors: 4 Yiping Qi, Department of Biology, East Carolina University, Greenville, North Carolina 27858; 5 Phone: 252-328-9764; Fax: 252-328-4178, Email: [email protected] 6 7 Yong Zhang, Department of Biotechnology, School of Life Sciences and Technology, University 8 of Electronic Science and...
متن کاملA CRISPR-Based Toolbox for Studying T Cell Signal Transduction
CRISPR/Cas9 system is a powerful technology to perform genome editing in a variety of cell types. To facilitate the application of Cas9 in mapping T cell signaling pathways, we generated a toolbox for large-scale genetic screens in human Jurkat T cells. The toolbox has three different Jurkat cell lines expressing distinct Cas9 variants, including wild-type Cas9, dCas9-KRAB, and sunCas9. We demo...
متن کاملExpansion of the CRISPR toolbox in an animal with tRNA-flanked Cas9 and Cpf1 gRNAs
We present vectors for producing multiple CRISPR gRNAs from a single RNA polymerase II or III transcript in Drosophila. The system, which is based on liberation of gRNAs by processing of flanking tRNAs, permits highly efficient multiplexing of Cas9based mutagenesis. We also demonstrate that the tRNA-gRNA system markedly increases the efficacy of conditional gene disruption by Cas9 and can promo...
متن کاملSharpening the LYM inequality
More detailed information about the s t ructure of Sperner families can be obtained by considering their level sequences. The level sequence of a family 4 , f ( ~ ) = {fi(Y)}, has f i (~) equal to the number of members of ~ with exac t ly ' / e l emen t s . Sperner 's theorem asserts tha t ~fi(2~)<_ ([~j) . A stronger restriction on the level i sequence was proved independent ly by Lubell, Yama...
متن کاملThe new genomic editing system (CRISPR)
Over the past decades, progression in genetic element manipulation, and consequently, the treatment of diseases has been remarkable. It is worth noting that these genetic manipulations perform at different levels, including DNA and RNA. The earlier genomic editing techniques, including MN, ZFN , TALEN , performing their functions by creating double-stranded breaks (DSBs), and after breakage, th...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: The CRISPR Journal
سال: 2020
ISSN: 2573-1599,2573-1602
DOI: 10.1089/crispr.2020.29114.rba